Dnmts and Tet target memory-associated genes after appetitive olfactory training in honey bees

نویسندگان

  • Stephanie D. Biergans
  • C. Giovanni Galizia
  • Judith Reinhard
  • Charles Claudianos
چکیده

DNA methylation and demethylation are epigenetic mechanisms involved in memory formation. In honey bees DNA methyltransferase (Dnmt) function is necessary for long-term memory to be stimulus specific (i.e. to reduce generalization). So far, however, it remains elusive which genes are targeted and what the time-course of DNA methylation is during memory formation. Here, we analyse how DNA methylation affects memory retention, gene expression, and differential methylation in stimulus-specific olfactory long-term memory formation. Out of 30 memory-associated genes investigated here, 9 were upregulated following Dnmt inhibition in trained bees. These included Dnmt3 suggesting a negative feedback loop for DNA methylation. Within these genes also the DNA methylation pattern changed during the first 24 hours after training. Interestingly, this was accompanied by sequential activation of the DNA methylation machinery (i.e. Dnmts and Tet). In sum, memory formation involves a temporally complex epigenetic regulation of memory-associated genes that facilitates stimulus specific long-term memory in the honey bee.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Corrigendum: Dnmts and Tet target memory-associated genes after appetitive olfactory training in honey bees.

This Article contains an error in the order of the Figures. Figures 3 and 4 were published as Figures 4 and 3 respectively. The correct Figures 3 and 4 appear below as Figs 1 and 2 respectively. The Figure legends are correct.

متن کامل

Olfactory memory formation and the influence of reward pathway during appetitive learning by honey bees.

Animals possess the ability to assess food quality via taste and via changes in state that occur after ingestion. Here, we investigate the extent to which a honey bee's ability to assess food quality affected the formation of association with an odor stimulus and the retention of olfactory memories associated with reward. We used three different conditioning protocols in which the unconditioned...

متن کامل

DNA Methylation Adjusts the Specificity of Memories Depending on the Learning Context and Promotes Relearning in Honeybees

The activity of the epigenetic writers DNA methyltransferases (Dnmts) after olfactory reward conditioning is important for both stimulus-specific long-term memory (LTM) formation and extinction. It, however, remains unknown which components of memory formation Dnmts regulate (e.g., associative vs. non-associative) and in what context (e.g., varying training conditions). Here, we address these a...

متن کامل

Honey Bees Modulate Their Olfactory Learning in the Presence of Hornet Predators and Alarm Component.

In Southeast Asia the native honey bee species Apis cerana is often attacked by hornets (Vespa velutina), mainly in the period from April to November. During the co-evolution of these two species honey bees have developed several strategies to defend themselves such as learning the odors of hornets and releasing alarm components to inform other mates. However, so far little is known about wheth...

متن کامل

Dnmts mediate neural processing after odor learning in the honeybee

DNA methyltransferases (Dnmts) epigenetic writers catalyzing the transfer of methyl-groups to cytosine regulate stimulus-specific olfactory long-term memory (LTM) formation and extinction in honeybees. The physiological relevance of their function in neural networks, however, remains unknown. Here, we investigated how Dnmts impact neuroplasticity in the bees’ primary olfactory center, the anten...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2015